A comprehensive panel of primary antibodies was used to detect various proteins: FOXO4, p53, Ser15-phospho-p53, p21 (cyclin-dependent kinase inhibitor 1A), p16 (cyclin-dependent kinase inhibitor 2A), 3-HSD, CYP11A1, CYP17A1 (cytochrome P450 family 17 subfamily A member 1), and several senescence-associated secretory phenotype (SASP) factors including IL-1, IL-1, IL-6, IL-10, TNF-, and TGF-
B12 injections are typically administered intramuscularlyusually in the upper arm or thigh
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Renal AOE status There was no significant difference in total SOD activity (Figure 1), and mRNA levels of CuZn-SOD or Mn-SOD (Table 2) between SHR and age-matched WKY